Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa
The EH_GH–pmKate2–N vector was expressed in cultured Escherichia coli cells, and the molecular weight of EH_GH protein was 24,558 Da. The EH_GH–pmKate2–N vector was introduced efficiently into the heads of the spermatozoa in the DMSO and positive control groups. Incubation of the spermatozoa with th...
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2021
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oai:localhost:DHTL-107272021-04-15T09:10:31Z Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa El-Sayed Shakweer, W.M. Hafez, Y.M. El-Sayed, A. Dessouki, S.M. Awadalla, I.M. Mohamed, M.I. Bovine growth hormone pmKate2–N expression vector Sperm uptake The EH_GH–pmKate2–N vector was expressed in cultured Escherichia coli cells, and the molecular weight of EH_GH protein was 24,558 Da. The EH_GH–pmKate2–N vector was introduced efficiently into the heads of the spermatozoa in the DMSO and positive control groups. Incubation of the spermatozoa with the vector caused a significant reduction in progressive motility compared to the negative control. https://bnrc.springeropen.com/articles/10.1186/s42269-019-0136-4#article-info 2021-04-15T09:10:31Z 2021-04-15T09:10:31Z 2019 BB 2522-8307 http://tailieuso.tlu.edu.vn/handle/DHTL/10727 en Bulletin of the National Research Centre, Vol 43:96 (2019) |
institution |
Trường Đại học Thủy Lợi |
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DSpace |
language |
English |
topic |
Bovine growth hormone pmKate2–N expression vector Sperm uptake |
spellingShingle |
Bovine growth hormone pmKate2–N expression vector Sperm uptake El-Sayed Shakweer, W.M. Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
description |
The EH_GH–pmKate2–N vector was expressed in cultured Escherichia coli cells, and the molecular weight of EH_GH protein was 24,558 Da. The EH_GH–pmKate2–N vector was introduced efficiently into the heads of the spermatozoa in the DMSO and positive control groups. Incubation of the spermatozoa with the vector caused a significant reduction in progressive motility compared to the negative control. |
author2 |
Hafez, Y.M. |
author_facet |
Hafez, Y.M. El-Sayed Shakweer, W.M. |
format |
BB |
author |
El-Sayed Shakweer, W.M. |
author_sort |
El-Sayed Shakweer, W.M. |
title |
Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
title_short |
Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
title_full |
Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
title_fullStr |
Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
title_full_unstemmed |
Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa |
title_sort |
uptake of exogenous bovine gh–pmkate2–n expression vector by rams spermatozoa |
publishDate |
2021 |
url |
http://tailieuso.tlu.edu.vn/handle/DHTL/10727 |
work_keys_str_mv |
AT elsayedshakweerwm uptakeofexogenousbovineghpmkate2nexpressionvectorbyramsspermatozoa |
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