Uptake of exogenous bovine GH–pmKate2–N expression vector by rams spermatozoa
The EH_GH–pmKate2–N vector was expressed in cultured Escherichia coli cells, and the molecular weight of EH_GH protein was 24,558 Da. The EH_GH–pmKate2–N vector was introduced efficiently into the heads of the spermatozoa in the DMSO and positive control groups. Incubation of the spermatozoa with th...
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| Định dạng: | BB |
| Ngôn ngữ: | English |
| Thông tin xuất bản: |
2021
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| Chủ đề: | |
| Truy cập trực tuyến: | http://tailieuso.tlu.edu.vn/handle/DHTL/10727 |
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| Tóm tắt: | The EH_GH–pmKate2–N vector was expressed in cultured Escherichia coli cells, and the molecular weight of EH_GH protein was 24,558 Da. The EH_GH–pmKate2–N vector was introduced efficiently into the heads of the spermatozoa in the DMSO and positive control groups. Incubation of the spermatozoa with the vector caused a significant reduction in progressive motility compared to the negative control. |
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