Sensitive Detection and Quantification of Burkholderia pseudomallei in Soil Samples Using Culture-Dependent and-Independent Approaches

Burkholderia pseudomallei, the causative agent of melioidosis, is a soil-dwelling Gram-negative bacterium. Although the bacterium has been known for a century, its worldwide distribution as well as its natural lifestyle still remains uncertain. Detection of B. pseudomalleiusing c...

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Tác giả chính: Trinh, Thanh Trung, Steinmetz, Ivo, Hetzer, Adrian
Định dạng: Working Paper
Ngôn ngữ:English
Thông tin xuất bản: NXB Nông Nghiệp 2011
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Truy cập trực tuyến:http://repository.vnu.edu.vn/handle/123456789/8934
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Tóm tắt:Burkholderia pseudomallei, the causative agent of melioidosis, is a soil-dwelling Gram-negative bacterium. Although the bacterium has been known for a century, its worldwide distribution as well as its natural lifestyle still remains uncertain. Detection of B. pseudomalleiusing culture-dependent and-independent methods has been previously reported. However, these methods have demonstrated either lack sensitivity or inaccurate quantification; therefore, environmental samples containing low numbers of B. pseudomalleiwill likely remain undetected or unquantifiable