Phân tích định tính và định lượng Flavonoid trong thân rễ Ngải đen (Kaempferia Parviflora Wall. ex. Baker ) bằng sắc ký lớp mỏng và phương pháp quang phổ / Đỗ Thị Hà, Vũ Thị Diệp, Nguyễn Thị Thu, Nguyễn Tiễn Dũng, Nguyễn Thị Thu, Trần Ngọc Lân
Flavonoids in the rhizomes of Kaempferia parviflora (Wall. ex. Baker) cultivated in Vietnam were determined: 5,7-dimethoxyflavone by thin-layer chromatography (TLC) and the total flavonoids by spectrophotometry, both with 5,7-dimethoxyflavone as reference. For qualitative TLC, the samples were prepa...
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| Ngôn ngữ: | vie |
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Vũ, Thị Diệp
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| Truy cập trực tuyến: | http://elib.ntt.edu.vn/Opac/DmdInfo.aspx?dmd_id=18631 |
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| Tóm tắt: | Flavonoids in the rhizomes of Kaempferia parviflora (Wall. ex. Baker) cultivated in Vietnam were determined: 5,7-dimethoxyflavone by thin-layer chromatography (TLC) and the total flavonoids by spectrophotometry, both with 5,7-dimethoxyflavone as reference. For qualitative TLC, the samples were prepared as follows: to 1.0 g of the ground material, 10 ml methanol was added, kept ultrasonicated for 1 h, filtered to get the filtrate for test solution. TLC was performed as: TLC plate - Silica gel 60 GF254; Developing solvent - a mixture of toluen – chloroform – aceton – methanol – acid formic (5:4:0,1:0.2:0.2, v/v/v/v/v). The total flavonoids were extracted with 96 % ethanol and Quantitatively determined by UV-spectrophotometry (265 nm) with 5,7-dimethoxyflavone as reference. The method was validated in terms of linearity (r2 = 0.9965), repeatability (RSD = 1.73 %) and accuracy (98.60 % - 100.55 %). The total flavonoids content of the samples collected from three provinces of Vietnam (Nghe An, Thanh Hoa and Son La) were found to be 4.10 – 5.91 % calculated on the dried materials.
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